9. Grow correct colony or colonies in LB+Amp overnight. Three is ideal since one should be correct without mutations which tend to occur most often at the junctions (regions of homology between fragments).

10. Perform miniprep(s) with the Qiagen Miniprep Kit.

11. Submit plasmid(s) for sequencing.

Ideally an assembly standard will use the same laboratory components (e.g. restriction enzymes and ligase) so that the same components can be used to assemble any two (or more) BioBricks together. This standard has one limitation in that custom primers need to be ordered for each individual assembly and hence requires different components. Nonetheless, this In-Fusion BioBrick Assembly method has been used to make several constructs and works well for myself and others in our lab. This method can be adapted to other plasmids in your favorite standard format and to assemble more than two BioBricks together. The method described here is a work-in-progress and the up-to-date method will be located here. Please document your success with using this method in the Notes section below!

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

List troubleshooting tips here.

You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.

Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding''''''*~~~~'''''':to the beginning of your tip.